Direct RT-qPCR saliva assays provide quick and easy sample analysis, enhance logistics, and lower sample handling and transportation costs. Meridian Bioscience claims to be the first company to provide a full solution for anyone working on a saliva-based molecular assay, whether liquid, lyophilized, or air-dried. The demand for direct saliva testing has surged with the rise in COVID-19 infections caused by the Omicron variant, as well as a quick increase in other respiratory diseases. Saliva is one of the easiest samples to collect and handle, but the high concentrations of PCR (polymerase chain reaction inhibitors) in the sample make implementing highly sensitive and reliable saliva-based diagnostics difficult. Outlook: Saliva is a non-invasive clinical sample that can be used to diagnose diseases such as cancer, HIV (human immunodeficiency virus), and respiratory infections. Using saliva samples and direct qPCR methods, both mixes speed up the development of diagnostic assays and enhance assay sensitivity. It’s ideal for creating lyophilized multiplex qPCR diagnostic tests at room temperature, as well as high-throughput, automated platforms with millisecond rehydration times. The mixtures are lyophilized to make ambient temperature-stable tests for point-of-care applications. As COVID-19 Sales Retreat, Diagnostic Firms Turn Attention to Core. Learn more about Meridian and the we provide. Meridian Bioscience Preliminary Fiscal Q2 Revenues Rise by up to 33 Percent. It can be used in liquid format or lyophilized. Meridian Bioscience Inc, is a global provider of molecular and immunological reagents and life science raw materials for diagnostic applications and diagnostic platforms and tests. The mix is ready-to-use, requiring only the insertion of primers and probes for further optimization. They’re designed to work with processed saliva or sputum samples for improved sensitivity and protocol flexibility. Nature of Disruption: Lyo-Ready qPCR master mix is a glycerol-free formulation that contains reaction buffer, Taq (Thermostable DNA) polymerase, dNTP (deoxyribose nucleotide triphosphate), lyo-excipients, and MgCl2 (Magnesium chloride). This RT-qPCR master mix with lyophilization compatibility is said to be ideal for high-throughput and point-of-care diagnostic assays. Concept: Ohio-based integrated life science company Meridian Bioscience has introduced two new master mixes, Lyo-Ready Direct DNA qPCR Saliva Mix and Lyo-Ready Direct RNA/DNA qPCR Saliva Mix, that can detect DNA and RNA in crude saliva samples.
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